Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber
نویسندگان
چکیده
منابع مشابه
detection of pvy, pvx, pvs, pva, and plrv on different potato varieties in turkey using das-elisa
this research was performed in order to study and diagnose potato virus y,potyvirus (pvy), potato virus x,potexvirus (pvx), potato virus s,carlavirus (pvs), potato virus a,potyvirus(pva) and potato leafroll luteovirus (plrv) on tubers and leaves of different potato varieties, namely, solea, safran, floris, proventa, milva, universa, lady olympia, vangogh, and marabel grown in afyon region of tu...
متن کاملDigoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
متن کاملDevelopment of RT-PCR based method for detection of potato virus Y in tobacco and potato
A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies tha...
متن کاملdetection of potato virus s (pvs) by serological and rt-pcr methods in khorasan razavi and hamedan provinces
abstract potato virus s is a member of the genus carlavirus in the family of flexiviridae, with curved filamentous particles 650×12 nm and positive single stranded rna genome. this virus is one of the most common viruses that infected all of potato varieties in the world. susceptible host species belong mainly to the families solanaceae, chenopodiaceae and amaranthaceae. potato virus s transmit...
متن کاملMolecular Detection of Potato Leaf Roll Polerovirus through Reverse Transcription Polymerase Chain Reaction in Dormant Potato Tubers
A Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique was applied for the detection of Potato leaf roll polerovirus (PLRV) in dormant potato tubers. A primer pair was designed from the coat protein-encoding fragment of the PLRV genome that amplified a 336-bp product. The amplified product was detected in nucleic acid preparations from leaves and tubers of 5 cultivars and from pur...
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ژورنال
عنوان ژورنال: Australasian Plant Disease Notes
سال: 2006
ISSN: 1833-928X
DOI: 10.1071/dn06017